DNA Construct Design Check Agent

A single base error in a construct design can cost weeks of lab work. Before ordering, this agent reads your construct map and translates the open reading frame, comparing it with the intended protein. It checks start and stop codons, tag placement and linkers, and looks for restriction sites that would cut the insert. It checks primer binding and melting temperatures and flags rare codons for the chosen host. Each problem gets a suggested fix, such as a silent change or a new primer, and it rechecks the revised design. The scientist approves every order. When sequencing comes back, it aligns the reads to the design and lists any differences. If the clone does not match, it suggests picking another colony. Edge case: a silent mutation added to remove a restriction site is accepted only if it does not create a rare codon.