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AI agent for biochemists

Compound Stock and Dilution Series Agent

Produce correct dilution series and pipetting sheets that stay within solubility and solvent limits

CreatingScience and research
See how it worksGet access
Compound Stock and Dilution Series Agent: what goes in, what the agent does and what you get

What it does

A dose-response experiment needs eight concentrations from several compounds. One stock is old, one precipitates at the top dose and one dilution step is miscalculated, so the curve looks odd and the experiment is repeated. This agent designs the series carefully. It builds a dilution scheme for each compound, checks solubility limits and stock age, generates pipetting sheets and verifies the final concentration at each point. If a point exceeds solubility, it redesigns the series with a lower top concentration or a different solvent, and recalculates. The scientist approves the sheets before use. Edge case: final DMSO exceeds the cell tolerance. The agent changes the intermediate dilution so the solvent stays constant.

How it works

Follow the arrows from top to bottom. The orange dashed arrow is the loop: when a check fails, the agent goes back and tries again.

Start and resultWhat it doesA check on its own workWaits for your OKGoes back and retries
Yes, continueYes, continueApprovedNoNo 1 STARTS WHEN Experiment planned 2 USES A TOOL Load compounds, stocks, solubility data and theplate layout 3 DOES Check stock age and concentration for each compound 4 DOES Design the dilution scheme to reach each targetconcentration 5 CHECKS THE RESULT Is every point below the solubility limit and thesolvent tolerance? If not: Lower the top concentration or change thesolvent and redesign. Back to step 4. 6 DOES Calculate volumes and generate pipetting sheets 7 CHECKS THE RESULT Do the calculated final concentrations match thetargets within 2 percent? If not: Correct the volumes and recalculate. Back tostep 4. 8 YOU APPROVE Scientist approves the sheets before use 9 USES A TOOL Release the sheets and record the stock lots used 10 RESULT Pipetting sheets and concentration table
Read the steps as a list
  1. Experiment planned
  2. Load compounds, stocks, solubility data and the plate layout
  3. Check stock age and concentration for each compound
  4. Design the dilution scheme to reach each target concentration
  5. Is every point below the solubility limit and the solvent tolerance?If not: Lower the top concentration or change the solvent and redesign. Back to step 4.
  6. Calculate volumes and generate pipetting sheets
  7. Do the calculated final concentrations match the targets within 2 percent?If not: Correct the volumes and recalculate. Back to step 4.
  8. Scientist approves the sheets before useThe agent waits here for your OK.
  9. Release the sheets and record the stock lots used
  10. Pipetting sheets and concentration table

How it decides

It chooses a dilution scheme that reaches each target within pipetting limits and keeps solvent constant. Points above solubility are removed or redesigned.

  • Stocks older than the stability limit are replaced or re-measured
  • Final solvent concentration must not exceed 0.5 percent unless approved
  • Pipetting volumes must be at least 2 microliters
  • Verify each final concentration against the target

Make it yours

Every agent is a starting point. You choose these settings for your own situation.

  • Maximum solvent percent (default 0.5)
  • Stock stability periods
  • Minimum pipetting volume (default 2 microliters)
  • Dilution factor
  • Plate formats

What keeps you in control

It always asks you first

  • Scientist approves the pipetting sheets before use

Hard limits

  • Never releases a sheet without approval
  • Always shows the calculation for each point

It stops when

  • Done: sheets approved and released
  • Stop: compound cannot reach target solubility, ask the scientist for options

Set it up

We guide you through the set-up, step by step

Members get the full set-up guide for this agent. No technical skills needed: you copy, paste and upload.

10 minto set it up in your AI
5 AIsChatGPT, Claude, Copilot, Gemini, Grok
  • One set of instructions to paste into your AI, with the clicks for ChatGPT, Claude, Microsoft 365 Copilot, Gemini and Grok
  • The agent then walks you through connecting your own data, one source at a time
  • A downloadable copy with the flow chart, the rules and the full guide
Get access to this agent

An example run

What happensFor 6 compounds across 8 concentrations up to 100 micromolar, the agent finds one compound is soluble only to 40. It sets that top point at 40 and shifts the series. A stock made 11 months ago is past its 6-month limit, so it asks for a fresh stock. It adjusts intermediate dilutions to keep DMSO at 0.1 percent. The scientist approves the sheets.

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